DarkDebugger for Dos16/32.

DD32.EXE is 32-bit version of DD.EXE.
Run DD32.EXE /? to display possible command line parameters.
You can run/debug sample program "vid.com" like below.

Running DD32 by command line:
dd32.exe vid.com

Running DD32 by installation (first install and next use "run.com" to load program):
dd32.exe
run.com vid.com

DD32 uninstall previously installed:
dd32.exe -u

DD32 installing in CONFIG.SYS:
DEVICE=DD32.EXE -e

TEST32.EXE is test file to run 32-bit dos extender program in V86 mode.